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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10054*  Cite 
Description

This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system. The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and orientation, and some coils are closely associated with the nuclear envelope, as is shown in this micrograph. The coils appear to stack in loose hexagonal arrays (see CIL:9301). The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).

Biological Sources
NCBI Organism Classification
Rana catesbeiana
Cell Type
barrier epithelial cell
Cellular Component
intermediate filament cytoskeleton
plasma membrane
Biological Context
Biological Process
intermediate filament cytoskeleton organization
Attribution
Names
John E. Michaels
Patricia A. Brown
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10054
Archival Resource Key (ARK)
ark:/b7295/w9cil10054
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Source of Contrast
Fbbi:00000415
Visualization Methods
uranyl salt
lead salt
osmium tetroxide
Processing History
unprocessed raw data
Dimensions
Spatial Axis Image Size Pixel Size
X 6121px 1nm
Y 4558px 1nm