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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10064*  Cite 
Description

Mammary epithelial MCF10A cells (immortalized normal mammary epithelial cells) transitioning from 2D to 3D collagen by grabbing and crawling on collagen-I fibers. scale bar = 50 microns. Time stamp Hrs:min:sec. Acquired in phase-contrast using a Plan Apo 20X 0.75 NA lens on a Nikon TE2000E inverted microscope. Celldance 2008, Honorable Mention Video

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
Cell Line
MCF 10A
Cellular Component
cell
extracellular matrix
Biological Context
Biological Process
cell migration
Attribution
Names
Ghassan Mouneimne (Harvard Medical School)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10064
Archival Resource Key (ARK)
ark:/b7295/w9cil10064
Imaging
Image Type
recorded image
Image Mode
phase contrast microscopy
Parameters Imaged
elastic scattering
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Methods
dispersed cells in vitro
cells in 3D matrix
Dimensions
Spatial Axis Image Size Pixel Size
X 540px 0.714µm
Y 410px 0.714µm
Time 150 seconds 12 hrs total