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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10105*  Cite 

Human retinal pigmented epithelial (RPE) cells labeled for clathrin-coated pits (green), focal adhesions (red) and nuclei (blue). RPE cells stabily expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) were plated directly on glass coverslides. After 72 hrs, cells were fixed for 2 min in a 1:1 mixture of 2% paraformaldehyde/0.5% Triton X-100, followed by a 30 min fixation in 4% paraformaldehyde. Cells were then stained with mouse monoclonal antibodies to paxillin, detected with goat anti-mouse Alexa 568. Nuclei were stained with DAPI. Image was collected on an Olympus IX71 inverted microscope coupled to a Hamamatsu ORCA-ER digital camera using a 60x 1.42 NA Plan Apo N objective (Olympus). The exposure times were 200, 500 and 70 msec for the red, green and blue channel, respectively. A xenon-arc lamp (Lambda LS stand-alone illuminator; Sutter Instrument) was used with adequat filters (Chroma). Bar: 5µm

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
retinal pigmented epithelial cell
Cellular Component
clathrin coat of coated pit
focal adhesion
clathrin light chain a
Marcel Mettlen
Sandra L. Schmid
The Scripps Research Institute
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Alexa Fluor 568
4',6-diamidino-2-phenylindole (DAPI)
primary antibody plus labeled secondary antibody
Processing History
3 color combine
Sample Preparation
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 1344px 0.027µm
Y 1024px 0.027µm
Channel Wavelength
1 350, 488, 568µm