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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10136*  Cite 
Description

Visualization of clathrin coated pit dynamics. Total internal reflection fluorescencec microscopy (TIRF-FM) was performed on live human retinal pigment epithelial (RPE) cells stably expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) using a 100x 1.45 NA objective (Nikon) mounted on a Nikon TE2000U inverted microscope (Nikon) coupled to a 14-bit mode operated Hamamatsu Orca II-ERG camera. This single channel TIR-FM movie, showing clathrin coated pit dynamics at the ventral plasma membrane, was acquired at a frame rate of 1s with a 70 msec exposure time during 10 min. Pixel size: 67 nm

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
human retinal pigmented epithelial cell
Cellular Component
clathrin coat of coated pit
clathrin light chain a
Biological Context
Biological Process
clathrin coated pit dynamics
Attribution
Names
Marcel Mettlen
Sandra L. Schmid
OTHER
The Scripps Research Institute
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10136
Archival Resource Key (ARK)
ark:/b7295/w9cil10136
Imaging
Image Type
recorded image
Image Mode
TIRF
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 613px 67µm
Y 991px 67µm