60 nm thick section of whole cell of budding yeast prepared by high-pressure freezing, freeze-substitution in acetone containing 1% glutaraldehyde and 0.1% uranyl acetate, and embedding in Lowicryl HM20 resin. Sections were stained with uranyl acetate and lead citrate, imaged with a Tecnai 12 TEM operated at 120 kV, and recoded with a Gatan Ultrascan 1000 CCD. Contrast was digitally adjusted. Micrograph illustrates the excellent preservation, especially of the of the plasma membrane and cell wall using this preparative technique.
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