Chicken erythrocyte nuclei were allowed to disperse in low salt, centrifuged through formaldehyde onto an EM grid, stained with uranyl acetate, and examined with the Madison 1MeV TEM at 40 KX in darkfield mode. This micrograph, showing tangled chromatin fibers of varying thickness, was recorded at a tilt angle of 45 degrees. The image is grouped with one of the same sample area recorded at a 55 degree tilt. A stereo view of the pair provides an oblique 3D impression of the specimen.
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