Figure 196 from Chapter 5 (Endoplasmic Reticulum) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A thin section through tibialis anterior of the mouse following fixation by glutaraldehyde solution lacking calcium, and postfixation with osmium-ferrocyanide solution. Skeletal tissue prepared this way results in staining of the etracellular space and T-tubules, but not the sacroplasmic reticulum. In this micrograph, two T-tubules can be seen in each sarcomere at the junctions of the A and I bands. Image by Michael Forbes. A PDF copy of the accompanying chapter is available on the ASCB’s BioEDUCATE website.