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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11845*  Cite 
Description

Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Fast retrograde flow still occurs in the lamellipodium, but the width of the lamellipodium is reduced. Retrograde flow in the lamellum is almost stopped. The cell was microinjected with X-rhodamine-conjugated actin and observed by spinning disk confocal microscopy. Fluorescent speckle time-lapse series were acquired on a spinning disk confocal microscope with a 100/1.4 NA Plan Apo objective lens. Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 5B of associated manuscript (J Cell Biol, 161:845-851, 2003).

Biological Sources
NCBI Organism Classification
Potorous tridactylus
Cell Type
epithelial cell
Cell Line
Pt K1 (NBL-3)
Cellular Component
actin cytoskeleton
Biological Context
Biological Process
actin polymerization or depolymerization
Molecular Function
PAK inhibition
Rac activation
Attribution
Names
Torsten Wittmann
Gary M. Bokoch
Clare M. Waterman-Storer
Published
J Cell Biol, 161:845-851, 2003
Pubmed
12796474
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11845
Archival Resource Key (ARK)
ark:/b7295/w9cil11845
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
X-Rhodamine
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 475px 0.131µm
Y 450px 0.131µm