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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11861*  Cite 
Description

Cultured retinal pigment epithelial cell were stably transfected with mCherry-clathrin light chain and EGFP-epsin. Live-cell time lapse images were collected on a Nikon TiE TIRF with a 100x1.49 NA objective (~67nm/pixel) using 120 ms exposures. This time series of mCherry-clathrin light chain is part of an image series that includes a single single dual color image and the time series for the EGFP-epsin as separate files.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
Cell Line
ARPE-19
Cellular Component
clathrin light chain
Attribution
Names
Allen Liu
Sandra L. Schmid
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11861
Archival Resource Key (ARK)
ark:/b7295/w9cil11861
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
TIRF
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
mCherryFP
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1344px 67nm
Y 1024px 67nm