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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11932*  Cite 

Four general zones of f-actin movement are seen in primary cultures of newt lung epithelial cells microinjected with X-rhodamine actin. At the leading edge and throughout the lamellipodium, f-actin speckles continuously appeared and moved towards the cell center. As speckles approached the junction between the lamellipodium and lamellum they usually disappeared, F-actin underwent slower retrograde flow in the lamellum, and F-actin generally moved forward in the cell body. Time-lapse FSM was performed on a spinning disk confocal microscope system. Light from a 50 mW Krypton-Argon ion laser (Melles Griot, OmniChrome) was delivered by a single-mode fiber optic (Point Source) to a Yokogawa spinning disk confocal scan-head (Ultra-View; PerkinElmer) on an inverted microscope (TE300 Quantum; Nikon). Images were collected by a 100x 1.4NA Plan-Apo DIC objective lens (Nikon) and captured with an Orca 2 camera (Hamamatsu). Images were collected in the ventral focal plane at 10 s intervals. Images were processed as follows: (1) background subtraction; (2) 3 x3 low pass filter; (3) unsharp mask filter. Corresponds to Fig 2E and video 2 in JCB , Volume 158, Number 1, July 8, 2002 31-37

Biological Sources
NCBI Organism Classification
Taricha granulosa
Cell Type
epithelial cell of lung
Cellular Component
actin cytoskeleton
Biological Context
Biological Process
actin filament polymerization
actin filament depolymerization
Wendy C. Salmon
Michael C. Adams
Clare M. Waterman-Storer
J Cell Biol, 158:31-37, 2002
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
fluorescent speckle microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
background subtraction, 3X3 low pass filter, unsharp mask
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 371px 0.119µm
Y 337px 0.119µm
Time 10 seconds 25min 34 s