Alternate header for print version

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11940*  Cite 

A live developing sea urchin embryo was imaged using differential contrast microscopy to enhance cellular details. Each minute a through focal series (Z-series) of micrographs was acquired to capture 3D information. In the movie, several Z-series captured about 1 hr apart are presented, providing a '4D' view of this stage in embryo development.

Technical Details

4-D images of a developing sea urchin gastrula (with DIC adjusted to show birefringence of developing spicules). Every 60 seconds, through-focal optical sections were acquired in rapid sequence ( 5 sec/stack of 104 frames) using stepper-motor-driven focusing controls on Shinya Scope 6, controlled by a Universal Imaging MetaMorph program . Out of the whole sequence, which lasted without interruption for 5 hours 20 minutes, four pairs of through-focal sequences separated approximately an hour apart are displayed here. See Article 42 (S. Inoue and TD Inoue 1986. Ann NY Acad Sci 483:392-404) in Inoue S 2008 Collected Works of Shinya Inoue World Scientific Publishing Co. Ltd. Hackensack, NJ pp 392-404.

Biological Sources
NCBI Organism Classification
Sea urchin embryo
Biological Context
Biological Process
Shinya Inoue
Inoue S 2008 Collected Works of Shinya Inoue World Scientific Publishing Co. Ltd. Hackensack, NJ pp 392-404.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
differential interference contrast microscopy
Parameters Imaged
optical path length gradient
Source of Contrast
boundaries between regions with different refractive index
Visualization Methods
visualization of contiguous regions
Data Qualifiers
processed data
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 720px ——
X 480px ——
Z 104px ——