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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11946^* Cite

Description

The movie shows two sequences that begin with an unfertilized Arbacia (sea urchin) egg in which the contents have been stratified using the centrifuge polarizing microscope (CPM) developed by S. Inoue. The main feature seen with the polarizing microscope is the appearance of a negatively birefringent layer of stacked membranes. Sperm are then introduced. A few seconds after sperm entry, this birefringence disappears as stacked membranes fragment. The egg also becomes lighter and rises.

Technical Details

See:S Inoue et al. 2001. Centrifuge polarizing microscope II. Sample biological applications. J Microsc 201:357-367.

Biological Sources

NCBI Organism Classification: Arbacia punctulata
Cell Type: oocyte

Biological Context

Biological Process: fertilization

Attribution

Names: Shinya Inoue
Published: S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL11946
Archival Resource Key (ARK): ark:/b7295/w9cil11946

Imaging

Image Type: recorded image
Image Mode: polarization microscopy
Parameters Imaged: retardance
Source of Contrast: form birefringence
Visualization Methods: visualization of contiguous regions
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	720px	——
X	480px	——