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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11969*  Cite 

The movie shows the behaviour of human erythrocytes suspended in a percoll gradient and exposed to centrifugal force in the centrifuge polarizing microscope developed by S. Inoue. Here, the contrast is generated by differential interference contrast (DIC). Erythrocytes are distorted in shape in the centrifugal field, the hemoglobin collecting at the lower part of the cell causing it to bulge. The movie shows the additions of single cells to a stack of cells (rouleaux). Upon joining a rouleaux, the erythrocytes instantly recover their normal undistorted biconcave shape.

Technical Details

See: J F Hoffman and S Inoue (2006). Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human and Amphiuma red blood cells. Proc Natl Acad Sci USA 103:2971-2976.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
Shinya Inoue
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
differential interference contrast microscopy
Parameters Imaged
optical path length gradient
Source of Contrast
boundaries between regions with different refractive index
Visualization Methods
visualization of contiguous regions
Data Qualifiers
processed data
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——