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CIL:11985*  Cite 

Time lapse of mCherry-tubulin by wide-field epifluorescence at the coverslip interface (left), mCherry-tubulin by TIRF (middle), and myosin-GFP by TIRF (right). The acquisition of Cherry-tubulin by TIRF was at 2s intervals, whereas the mCherry-tubulin by epifluorescence and myosin-GFP TIRF were at 36 and 10 s intervals, respectively. Video corresponds to Fig 3A and video 2 from J Cell Bio 186:727-738, 2009. Note that this video is the wild type control for another video in the series that shows the same biological process in the presence of kinesin-6 RNAi. Total internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491-nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491/561/633rpc) and changed the emission filter (ET525/50 or ET595/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405/488/561/635; Semrock, Inc.). Images were typically captured every 2-3 s with a 50-200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
epithelial cell
Cell Line
Cellular Component
myosin regulatory light chain
Biological Context
Biological Process
mitotic anaphase
Ronald D. Vale
James A. Spudich
Eric R. Griffis
J Cell Bio 186:727-738, 2009
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 1252px 0.128µm
Y 394px 0.128µm
Channel Wavelength
1 488, 561nm
Time 2 seconds