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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12022*  Cite 
Description

Movie of meiosis I (diakinesis through telophase)in primary spermatocytes of the crane-fly Nephrotoma suturalis that have been experimentally flattened. Flattening prevents completion of cytokinesis. Time-lapse DIC microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 2 min for 180 min using a QImaging Retigo EXi CCD camera. A movie of the time series is based is included in this grouped set.

Biological Sources
NCBI Organism Classification
Nephrotoma
Nephrotoma suturalis
Cell Type
primary spermatocyte
Cellular Component
spindle
nuclear chromosome
Biological Context
Biological Process
male meiosis chromosome segregation
Attribution
Names
James R. LaFountain
Rudolf Oldenbourg
Published
LaFountain JR, et al. (2004) Mol Cell Biol 15, 5724-5732
Pubmed
15469981
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12022
Archival Resource Key (ARK)
ark:/b7295/w9cil12022
Grouping This image is part of a group.
Imaging
Image Type
charge coupled device (CCD)
Image Mode
differential interference contrast microscopy
Parameters Imaged
UV/visible/IR photons
Source of Contrast
differences in refractive index
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 678px 87nm
Y 518px 87nm
Time 120 seconds 180