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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12083*  Cite 
Description

A high resolution view of the radial arm of the Contractile Vacuole Complex or CVC each contain a collecting canal enclosed by a microtubular ribbon split lengthwise into several smaller ribbons, a meshwork of smooth spongiome and, peripheral to this, the bundles of tubules of the decorated spongiome. The microtubules along the collecting canal are immunogold-labeled with anti-b-tubulin as are some single microtubules in the area. TEM taken on 7/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 7,900X.

Technical Details

Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with a Nikon Coolscan 9000ED. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium tetraurelia
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
contractile vacuole
contractile vacuolar membrane
cytoplasmic microtubule
Biological Context
Biological Process
contractile vacuole organization
cytoplasm organization
Attribution
Names
Richard Allen (University of Hawaii)
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12083
Archival Resource Key (ARK)
ark:/b7295/w9cil12083
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
gold
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in vitreous ice embedment
microtome-sectioned tissue
monoclonal antibody
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 5418px 1.8nm
Y 6027px 1.8nm