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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12287*  Cite 
Description

Behaviors of the microtubule anchoring factor RFP-LL5α (red) and microtubules (green) in living epithelial cells undergoing random migration. During the time-lapse imaging, the cells divided into two daughter cells. Cortical patches of RFP-LL5α appear when cells become attached to the glass substrate, but disappear when the cells become detached at the onset of cell division. The patches reappear soon after the cells adhere to the glass again. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This is the original data file for Video 4 from J Cell Biol (2010) 189 (5):901-917.

Technical Details

MCF-10Aeco cells expressing GFP-α-tubulin and RFP-LL5α were seeded on glass-bottomed dishes at a low density and cultured overnight. Time-lapse images of the cells were collected at 2.5-min intervals for 8 h 6 min using a DeltaVision Core Systemdriven by SoftWoRx software (Applied Precision) equipped with an IX70 microscope with a Plan-Apochromat 100× NA 1.40 oil immersion objective (Olympus), a cooled charge-coupled device camera (CoolSNAP HQ2; Photometrics) and a CO2 incubator (Tokai Hit Co., Ltd.). GFP, Ex, 470; Em, 525; RFP, Ex, 572, Em, 632.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
Cell Line
MCF 10A
Cellular Component
microtubule
cell cortex part
Attribution
Names
Azusa Hotta
Tomomi Kawakatsu
Tomoya Nakatani
Toshitaka Sato
Chiyuki Matsui
Taiko Sukezane
Tsuyoshi Akagi
Tomoki Hamaji
Ilya Grigoriev
Anna Akhmanova
Yoshimi Takai
Yuko Mimori-Kiyosue
Published
J. Cell Biol. 2010. 189(5):901-917
Pubmed
PMID:20513769
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12287
Archival Resource Key (ARK)
ark:/b7295/w9cil12287
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
DsRed-monomerFP
EGFP
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 432px 0.127µm
Y 399px 0.127µm
Time 150 seconds 196