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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12289*  Cite 
Description

To determine the extent to which extracellular matrix proteins colocalize with the microtubule-anchoring factor LL5β, MCF-10A epithelial cells were immunostained with antibodies to the secreted autocrine laminin-5 (green), the laminin receptor integrin α6 (blue) and LL5β (red). Analyses suggest that LL5s are colocalized with activated pools of integrins associated with laminin-5. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file for Fig. 3 Panel D-b from J Cell Biol (2010) 189 (5):901-917.

Technical Details

MCF-10A epithelial cells were fixed with 1:1 mixture of methanol/acetone (-20° C, 10 min), permeabilized with 0.5% Triton in PBS, and immunostained sequentially with primary, then secondary antibodies. LL5β (Cy5, 633 nm laser), laminin-5 (rhodamine red-X, 543 nm laser)) and integrin α6 (Cy2, 488 nm laser, and observed using a confocal laser-scanning microscope system. For additional details see: J. Cell Biol. 2010. 189(5):901-917.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
Cell Line
MCF 10A
Cellular Component
microtubule
cell cortex part
Attribution
Names
Azusa Hotta
Tomomi Kawakatsu
Tomoya Nakatani
Toshitaka Sato
Chiyuki Matsui
Taiko Sukezane
Tsuyoshi Akagi
Tomoki Hamaji
Ilya Grigoriev
Anna Akhmanova
Yoshimi Takai
Yuko Mimori-Kiyosue
Published
J. Cell Biol. 2010. 189(5):901-917
Pubmed
PMID:20513769
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12289
Archival Resource Key (ARK)
ark:/b7295/w9cil12289
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
Cy2
Cy5
X-Rhodamine
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
methanol fixed tissue
permeabilized tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 0.07µm
Y 1024px 0.07µm