To determine the extent to which extracellular matrix proteins colocalize with the microtubule-anchoring factor LL5s (LL5α), MCF-10A epithelial cells were immunostained with antibodies to laminin receptors α3 (green), α6 (blue) and LL5s (red). Analyses suggest that LL5s are colocalized with activated pools of integrins associated with laminin-5. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file for Fig. 3 Panel D-c from J Cell Biol (2010) 189 (5):901-917.
MCF-10A epithelial cells were fixed with 1:1 mixture of methanol/acetone (-20° C, 10 min), permeabilized with 0.5% Triton in PBS, and immunostained sequentially with primary, then secondary antibodies and laminin receptor (Cy5, 633 nm laser), laminin receptor α6 (Cy2, 488 nm laser) and laminin receptor α3(rhodamine red-X, 543 nm laser), and observed using a confocal laser-scanning microscope system. For additional details see: J. Cell Biol. 2010. 189(5):901-917
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