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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12293*  Cite 
Description

Colocalization of the microtubule anchoring factor LL5α (red) with integrin α6 (blue) and laminin (green) in the epithelium of mouse small intestine. LL5α is localized to the basal cell cortex attached to the laminin BMs. Overall findings from this publication showed that signaling from laminin-integrin associations play a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file from Fig. 4D, J Cell Biol (2010) 189 (5):901-917.

Technical Details

Small intestine from C57BL6/J strain mice (8 wk of age) was prepared by perfusion fixation, processed as described previously (Nakatani et al., 2004), and immunostained for laminin (Cy2, 488 nm laser), LL5a (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
epithelial cell
Cellular Component
microtubule
basal cortex
microvillus
basement membrane
Attribution
Names
Azusa Hotta
Tomomi Kawakatsu
Tomoya Nakatani
Toshitaka Sato
Chiyuki Matsui
Taiko Sukezane
Tsuyoshi Akagi
Tomoki Hamaji
Ilya Grigoriev
Anna Akhmanova
Yoshimi Takai
Yuko Mimori-Kiyosue
Published
J. Cell Biol. 2010. 189(5):901-917
Pubmed
PMID:20513769
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12293
Archival Resource Key (ARK)
ark:/b7295/w9cil12293
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
Cy2
X-Rhodamine
Cy5
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 0.45µm
Y 1024px 0.45µm


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