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CIL:12317*  Cite 

Chick embryos have been used for the developmental study of vertebrates. However, the continuous and long-term observation of the same embryo is difficult, because development tends to be interrupted when a large window is made through the eggshell. To overcome this problem, the authors of this video established a continuous observation system for developing chick embryos. In this system, a window is made through the eggshell for observation, and then the egg is returned to the incubator. Time-lapse video images are taken and re-constituted into an animation. Fertilized white Leghorn chicken eggs (Miyake Hatchery, Inc., Japan) were incubated at 37.7oC in a humidified incubator (p-008; Showa Furanki, Inc., Japan) for 2 days. A shell cap with a diameter of 3 cm at the sharp end of each egg was cut out with a file to make a window, and the hole was sealed immediately with poly-vinylidene-chloride film (Asahi Kasei Home Products Corporation, Japan). The eggs were then incubated again under illumination for 18 days. Images were taken every hour by a video microscope (VH-5000C; Keyence, Japan) and assembled into an animation.

Biological Sources
NCBI Organism Classification
Gallus gallus
Biological Context
Biological Process
in ovo embryonic development
Ai Shima
Masaya Watanabe
Takuto Hirabayashi
Ryoichi Matsuda
University of Tokyo, Tokyo
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
bright-field microscopy
Parameters Imaged
absorption of illumination
Source of Contrast
differences in intrinsic optical density
Visualization Methods
visualization of contiguous regions
Processing History
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 320px 113.6µm
Y 240px 113.6µm
Time 3600 seconds 18 days