A view of the cytopharynx and its nascent food vacuole forming region. The flattened pharyngeal vesicles that may contain monolayers or bilayers of lipids seem to be equivalent to the discoidal vesicles of Paramecium that provide membrane for food vacuole growth. They approach and fuse with the cytopharyngeal membrane between the lamellae (cytopharyngeal ribbons). TEM taken on 7/29/67 by R. Allen with Philips 200 operating at 60kV. Neg. 9,000X. Bar = 0.5µm.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. There is a high resolution version of this image in the library (CIL:39114) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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