Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12410^* Cite

Description

Localization and intracellular motility of myosin IIA (MIIA) in protrusive regions of migrating CHO.K1 cells. Arrowheads point to locations where representative MIIA filaments form. This Video corresponds to Fig. 6 A and video 3 in JCB 183:543-554 , 2008. Images of GFP-MIIA were collected every 4 s using TIRF microscopy. Images were acquired in an inverted microscope (model IX70;Olympus) with 488 nm excitation. A dichroic mirror (HQ485/30) was used for GFP-labeled cells. Images were acquired with a charge-coupled device camera (Retiga Exi; Qimaging) and analyzed using MetaMorph software (MDS Analytical Technologies). 10 frames/s shown.

Biological Sources

NCBI Organism Classification: Cricetulus griseus
Cell Type: epithelial cell
Cell Line: CHO-K1
Cellular Component: myosin IIA

Biological Context

Biological Process

Attribution

Names: Miguel Vicente-Manzanares; Margaret A. Koach; Leanna Whitmore; Marcelo L. Lamers
Published: JCB 183:543-554 , 2008
Pubmed: 18955554

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12410
Archival Resource Key (ARK): ark:/b7295/w9cil12410

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: TIRF
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	364px	0.076µm
Y	284px	0.076µm

Time	4 seconds