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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12411*  Cite 
Description

Localization and intracellular motility of myosin IIB (MIIB) in protrusive regions of migrating CHO.K1 cells. This Video corresponds to Fig. 6 B and video 4 in JCB 183:543-554 , 2008. Images of GFP-MIIB (green) and RFP-actin (magenta) were collected every 4 s using TIRF microscopy with 10 frames/s shown. Images were acquired in an inverted microscope (model IX70;Olympus). GFP and RFP were excited using the 488-nm laser line of an Ar ion laser and the 543-nm laser line of a He-Ne laser (Melles Griot), respectively. For dual GFP- mRFP/mCherry/mOrange, a dual-emission filter (z488/543) was used. Images were acquired with a charge-coupled device camera (Retiga Exi; Qimaging) and analyzed using MetaMorph software (MDS Analytical Technologies).

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
epithelial cell
Cell Line
CHO-K1
Cellular Component
myosin IIB
actin cytoskeleton
Biological Context
Biological Process
cell migration
Attribution
Names
Miguel Vicente-Manzanares
Margaret A. Koach
Leanna Whitmore
Marcelo L. Lamers
Published
JCB 183:543-554 , 2008
Pubmed
18955554
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12411
Archival Resource Key (ARK)
ark:/b7295/w9cil12411
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
TIRF
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
mRFP1
Processing History
unprocessed raw data
color combine
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 364px 0.077µm
Y 284px 0.077µm
Time 4 seconds