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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12416*  Cite 
Description

The images in this group show electron micrographs of epidermal and mesophyll cells in thin sections of maize leaves infected with Maize mosaic virus (MMV, Rhabdoviridae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. This tif image is of a mesophyll leaf cell from a maize plant infected with Maize mosaic virus (MMV), and is identical to the labeled jpg file CIL:16309. MMV virions, budding through the inner nuclear membrane, are accumulating between this membrane and the outer nuclear membrane as well as in intracytoplasmic vesicles, sometimes forming crystalline arrays. Detailed methods: Pieces of leaves were processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. See E.-D. Ammar et al. (2005) J. Phytopathology, 153:129-136.

Biological Sources
NCBI Organism Classification
Maize mosaic virus
Zea mays
Cell Type
plant cell
Cellular Component
host cell nuclear membrane
virion
host cell cytoplasmic vesicle
Biological Context
Biological Process
viral reproduction
Attribution
Names
El-Desouky Ammar
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12416
Archival Resource Key (ARK)
ark:/b7295/w9cil12416
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
osmium tetroxide
lead salt
uranyl salt
Data Qualifiers
raw, unprocessed data
Dimensions
Spatial Axis Image Size Pixel Size
X 1181px ——
Y 818px ——