Alternate header for print version

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12576*  Cite 

A wild-type mouse embryos on embryonic day 9.5 was isolated, fixed in 3.5% para-formaldehyde in PBS, rinsed in PBS, and was imaged with a prototype optical coherence microscopy (OCM) imaging system. With OCM an optical beam is directed at the tissue, and a small portion of this light that reflects from sub-surface features is collected. Note that most light is not reflected but, rather, scatters. Scattered light can be filtered out using optical coherence. Only the reflected (non-scattered) light is coherent (i.e., retains the optical phase that causes light rays to propagate in one or another direction). In the OCM, an optical interferometer is used to detect only coherent light. The OCM data was acquired over a 400um x 400um field of view at 2 frames per second. The spacing between each 3D-OCM slice is 5um along the depth dimension. The OCM data on the mouse embryo confirmed that myocardial-endocardial radial associations exist in the intact ED 9.5 looping mouse heart.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Line
Biological Context
Biological Process
myocardial-endocardial radial associations
Chao Zhou
Michael W. Jenkins
Barbara Garita
Mingda Han
Michael VanAuker
Andrew M. Rollins
Michiko Watanabe
James G. Fujimoto
Kersti K. Linask
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
optical coherence microscope
Parameters Imaged
reflected light
Source of Contrast
interferometric coherence
Visualization Methods
inherent scattering
Processing History
unprocessed raw data
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 700px 0.57µm
Y 700px 0.57µm
Z 102px 5µm