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CIL:12577*  Cite 

A contraction wave circulating across a stage 18 medaka embryo over a 2.5-min interval (30 sec between each image). The embryo is ensheathed by a chorion which possesses numerous filamentous villi. The spherical objects in the image are oil droplets located within the embryo's yolk cell. Over a course of 2.4 hr, 55 contraction waves circulated across the embryo. The contraction waves had an average period of 2.7 midcontraction cycle. Note that during the contraction cycle, the oil droplets in the yolk cell have rotated approximately 10" counterclockwise with respect to the stationary chorion. Time-lapse phase recordings of contracting embryos and explanted periderms were made using a Nikon Diaphot coupled to a Newvicon video camera (Hamamatsu Corp., Middlesex, NJ). Phase-contrast optics (10x/0.3 NA or 20x/0.4 NA objectives) and a halogen light source were used to record macroscopic movements of whole embryos or explants. All experiments were conducted at an ambient room temperature of approximately 22°C. Related to image 1 in J Zoology 273:118-129 (1995).

Biological Sources
NCBI Organism Classification
Oryzias latipes
Cell Type
Biological Context
Biological Process
contraction wave
Joanne Z. Simon
Mark S. Cooper
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
phase contrast microscopy
Parameters Imaged
elastic scattering
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 160px 7.27µm
Y 120px 7.27µm
Time 30 seconds 2.5 min