The flagellar pocket of a Trypanosoma brucei bloodstream form cell, with short flagellum. The image shows a new flagellum starting to grow from the flagellar pocket, in preparation for the next cell division. Cells were high pressure frozen and freeze substituted (1hr freeze substitution in 2% uranyl acetate in acetone and 8% methanol). An 80nm thick section was stained for 5 minutes with uranyl acetate, one minute with lead citrate and viewed using a Philips CM100 transmission electron microscope. The image was captured with an Advantage HS-B camera (2000 x 2000 pixels; AMT, Danvers, MA, USA). Contrast adjusted using Photoshop. Magnification indicated by scale bar.
|Spatial Axis||Image Size||Pixel Size|