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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12617*  Cite 
Description

High resolution image of an early endosome in planar view that contains HRP-reaction product. This cell was exposed to HRP for 6 min. and then fixed. This early endosome had two small coated evaginations arising from its rim and also had two large HRP-containing uncoated vesicles docked next to it presumably ready to fuse with it (for this picture and a serial section showing these two vesicles and the second evagination see Fig. 14C in Allen, Schroeder and Fok, J. Cell Sci. 101:449-461, 1992). Mitochondria have an endogenous peroxidase activity. This image was adapted with permission from Fig. 14. TEM taken on 10/20/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cell cortex
endosome
Biological Context
Biological Process
endosome organization
endosome to lysosome transport
Attribution
Names
Richard Allen (University of Hawaii)
Published
J. Cell Sci. 101:449-461, 1992
Pubmed
1629255
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12617
Archival Resource Key (ARK)
ark:/b7295/w9cil12617
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
horseradish peroxidase
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 5360px 0.76nm
Y 4938px 0.76nm