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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13135*  Cite 
Description

The somatic basal bodies along the sides of the cytoproct ridge have bundles of microtubules extending into the endoplasm from near their proximal ends. These bundles make contact with the spent vacuole and aid its movement to the cytoproct. The plasma membrane and alveolar sacs were ruptured in this cell during preparation for electron microscopy. TEM taken on 6/13/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,000X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12350) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cytoproct
cortical microtubule cytoskeleton
cytoplasm
Biological Context
Biological Process
defecation
cortical cytoskeleton organization
Attribution
Names
Richard Allen (University of Hawaii)
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13135
Archival Resource Key (ARK)
ark:/b7295/w9cil13135
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2928px ——
Y 3370px ——