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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13160*  Cite 
Description

Topographical regulation of keratinocyte migration. 2D, 3D, and 1D fibrillar migration of human epidermal keratinocytes. 2D matrices were constructed by uniform coating with extracellular matrix (ECM). 3D surfaces were constructed according to Science. 294: 1708–1712 (2001). 1D surfaces were made by using a 2 photon confocal microscope to pattern a polyvinyl alchohol (PVA) coated coverslip. Movie is video 4 from JCB 2009, 184:481-490

Technical Details

Following gluteraldehyde quenching, ECM proteins were absorbed onto the PVA surface. Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and maintained cells at 37°C with 10% CO2. Images were collected every 4 min and shown at 15 frames per second.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
keratinocyte
epidermal cell
Cellular Component
cell
Attribution
Names
Andrew D. Doyle
Francis W. Wang
Kazue Matsumoto
Kenneth M. Yamada
Published
JCB 2009, 184:481-490
Pubmed
19221195
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13160
Archival Resource Key (ARK)
ark:/b7295/w9cil13160
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
custom-made smoothing, sharpening, and convolution filters using MetaMorph software
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 900px 0.45µm
Y 300px 0.45µm
Time 240 seconds