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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13377*  Cite 
Description

z-focal series through Hela cell in metaphase illustrating MCAK localization in Bod-1siRNA cells. Cells were transfected with control (this image) or Bod1 (CIL: 13378) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.

Technical Details

Antibodies were rabbit anti-HEC1 (green) antibody (Abcam) used at 1:1,000. and sheep anti-MCAK (red) (Andrews et al., 2004) used at 1 μg/ml, and DNA (blue). Fluorescently labeled secondary antibodies were all obtained from Jackson ImmunoResearch Laboratories. Corresponds to Fig 5B in JCB vol. 179 no. 2 187-197.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
HeLa S3
Cellular Component
chromosome, centromeric region
DNA
HEC1
MCAK
Biological Context
Biological Process
mitosis
Attribution
Names
Iain M. Porter
Sarah E. McClelland
Guennadi A. Khoudoli
Christopher J. Hunter
Jens S. Andersen
Andrew D. McAinsh
J. Julian Blow
Jason R. Swedlow
Published
JCB vol. 179 no. 2 187-197
Pubmed
17938248
Link
Journal of Cell Biology
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13377
Archival Resource Key (ARK)
ark:/b7295/w9cil13377
Grouping This image is part of a group.
Sample Preparation
Methods
chemically fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 355px 0.0629µm
Y 381px 0.0629µm
Z 28px 0.2µm