Alternate header for print version

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13389*  Cite 

This image is one of a group of 4 images that supports the conclusion that hRio2 kinase activity is required for cytoplasmic 18S-E pre-rRNA processing. This image shows the distribution of all 18S rRNA precursors (white), via fluorescence in situ hybridization (FISH) with a 5' ITS1 probe, upon depletion of a protein important in rRNA processing, hRio2, and subsequent attempted rescue by transfection with an EGFP-tagged kinase-dead version of hRio2, in Hela cells. Depletion of hRio2 leads to the accumulation of 18S-E pre-rRNA in the cytoplasm (white), and this is rescued only by WT hRio2 (a companion image in this group), but not by hRio2 protein that lacks kinase activity, seen here in green. Note that the 5' ITS1 probe shows the localization of all 18S rRNA precursors, but only 18S-E pre rRNA is detected in the cytoplasm. HeLaK cells were transfected with Rio2-d siRNA and then the rescue construct was transfected after 48 h of RNAi. Cells were fixed after 72 h of RNAi, followed by FISH analysis. In the set of four images in this group, FISH pictures were processed in parallel by enhancing levels, followed by setting gamma correction to 0.75.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
Cellular Component
preribosome, small subunit precursor
Biological Context
Biological Process
maturation of SSU-rRNA
Ivo Zemp
Thomas Wild
Marie-Francoise O'Donohue
Franziska Wandrey
Barbara Widmann
Pierre-Emmanuel Gleizes
Ulrike Kutay
JCB 2009, 185:1167-1180
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
distribution of a specific nucleic acid sequence
Visualization Methods
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 512px 268nm
Y 512px 268nm