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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13469*  Cite 

Transmission electron micrograph of Drosophila nephrocyte garland cell from ema[1] mutant: high magnification view. ema mutant garland cells are filled with very large electron-lucent alpha and electron-dense beta vacuoles, many of which are 5–10 microns in diameter, whereas the largest vacuoles in wild type are ∼2 microns in diameter. In wild type, both types of vacuoles usually contain a single aggregate of electron-dense granular material that appears to be attached to the limiting membrane. However, the large vacuolar compartments in the mutant contain numerous electron-dense granular structures, suggesting they arise from homo-/heterotypic fusions of alpha and beta vacuoles. Samples for TEM were fixed for 1 h at 4°C in 2% paraformaldehyde, 2% glutaraldehyde, and 1% tannic acid in 0.1 M cacodylic acid buffer, pH 7.2. Then, the samples were postfixed in 1% OsO4 in 0.1 M cacodylic acid buffer, pH 7.2, for 1 h at room temperature, stained en bloc with 1% uranyl acetate, dehydrated in a grade series of ethanol and propylene oxide, and embedded in epon resin (Electron Microscopy Sciences). Blocks were sectioned in an ultramicrotome (RMC Products) at ∼70-nm thickness with a Delaware Diamond knife and post-stained for 1 h in Reynolds lead citrate and uranyl acetate. Electron micrographs were taken on a transmission electron microscope (H-7500; Hitachi) using an AMT camera system. Mag: 15000x. Image corresponds to Figure 2C, right ema[1] panel in Kim et al. J Cell Biol. 188: 717-734. 2010. This is a high magnification from same region as micrograph CIL# 13468.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
garland cell
Cell Line
Cellular Component
early endosome
late endosome
Biological Context
Biological Process
Sungsu Kim
Yogesh P. Wairkar
Richard W. Daniels
Aaron DiAntonio
J Cell Biol. 188: 717-734. 2010
PMID: 20194640
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
charge coupled device (CCD)
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
lead salt
osmium tetroxide
Data Qualifiers
raw, unprocessed data
Spatial Axis Image Size Pixel Size
X 1024px ——
Y 1184px ——