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CIL:13524*  Cite 

A confocal Z-stack of neurons in postnatal hippocampus, expressing a GFP-tagged knockdown construct for the microRNA miR-137 (green, sh-miR-137), a marker of neuronal differentiation, çand BrdU (red) to localize total cells and newly-generated cells respectively. miR-137 regulates a core transcription factor in stem cell renewal. Data from these experiments suggest that high levels of miR-137 promotes neuronal stem cell proliferation, but repressed neuronal differentiation. This image is original data from Fig. 5 of Szulwach et al.(2010) Cross talk between microRNA and epigenetic regulation in adult neurogenesis, J. Cell Biol. Vol. 189:127–141.

Technical Details

A retroviral vector containing sh-miR-137 under a U6 promoter and EGFP under a chicken actin promoter was prepared, and the virus containing the construct was injected into the dentate gyrus of adult mice in vivo. BrdU was injected twice/day (50,g/kg i.p.) for a total of 7 injections beginning immediately after viral grafting. After 1 week, animals were anesthetized, perfused with 4% paraformaldehyde, brains removed and postfixed, equilibrated in 30% sucrose, and sectioned at a thickness of 40µm with a sliding microtome. Infected cells were localized using chicken-α-GFP (Invitrogen) and rat-α-BrdU (Abcam), secondaries were α-chicken Alexa Fluor 488 and goat-α-rat Alexa Fluor 647 (both from Invitrogen). Confocal z-stacks were acquired at 1 µm steps using a Nikon TE2000 with equipped with a spinning disc confocal with an oil immersion 40X 1.3 NA objective (Zeiss). For additional details see: J. Cell Biol. Vol. 189:127–141.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
neuronal stem cell
Cellular Component
Keith E. Szulwach
Xuekun Li
Richard D. Smrt
Yujing Li
Yuping Luo
Li Lin
Nicholas J. Santistevan
Wendi Li
Xinyu Zhao
Peng Jin
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
formaldehyde fixed tissue
permeabilized tissue
Relation To Intact Cell
microtome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 1024px ——
Y 1024px ——
Z 23px 1µm