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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13544*  Cite 

Helix Pomatia Lectin (HPL) (green) staining at the Golgi (Giantin) (red) in unstimulated HeLa cells. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. Image also includes anti-GalNacT1 staining (gray). HeLa cells were serum starved overnight in DME (noFBS). Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100). Primary antibody staining followed the manufacturer’s instructions. Cells were subsequently stained for 15–30 min with secondary Alexa Fluor–conjugated antibodies (Alexa 488 for anti-Giantin, Alexa 594 for anti GalNac-T1), Hoechst (blue) and Alexa 647-conjugated-HPL was added during secondary antibody incubations. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with fluorescence excitation at 488 nm, 561 nm, and 633 nm and either a 60x objective (U Plan Super Apochromatic [UPLSAPO]; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 1A in J Cell Biol. 189: 843-858. 2010. Images in Fig 1 include CIL#s 13543, 13544, 13545, 13546.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
cervical carcinoma
Cell Line
Cellular Component
Golgi stack
integral to membrane
Golgi cisterna membrane
David J. Gill
Joanne Chia
Jamie Senewiratne
Frederic Bard
J Cell Biol. 189: 843-858. 2010.
PMID: 20498016
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 1024px 0.207µm
Y 1024px 0.207µm
Z 1px 2.83µm