The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The myoblasts in this image were transfected with the locked nucleic acid (LNA) anti-miR-1 and treated with 25 nM trichostatin A (TSA), an inhibitor of histone deacetylase (HDAC), the day before switching to differentiation medium. The effect of miR-1was rescued by TSA. This image is one of different treatments in Figure 6B from JCB 189: 1157-1169, 2010. See also CIL: 13585, 13586, 13588.
The image was taken by leica DMI 4000B microscope equipped with a QImaging RETIGA Exi camera and Leica 10X/0.22 lens. Myosin heavy chain (MHC) expressed in myotubes was labeled by MF-20 mouse antibody followed by FITC-anti-mouse IgG secondary antibody. Nuclei were stained by DAPI. MHC and nuclei were pseudocolored in green and blue, respectively.
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