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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13595*  Cite 
Description

Mouse hind limb tibialis anterior (TA) muscles were injected with BaCl2 followed by daily systemic administration of rapamycin (Rap) starting 7 days after injury. The animals were sacrificed at different days, and the injured muscles were dissected and cryosectioned followed by hematoxylin (blue) and eosin (pink to red) staining. This image shows the damage muscle tissue section one day after the injury as a control before regeneration. The muscle tissue was stained by eosin. Dark red patches were the remaining live muscle fibers and the white to pink void was the dead tissue. Nuclei (blue) were stained by hematoxylin. This image corresponds to Fig 10B from JCB 189: 1157-1169, 2010. See also CIL: 13596, 13597, 13598, 13599, and 13600.

Technical Details

TA muscles were isolated by dissection, frozen in liquid nitrogen–cooled 2-methylbutane, and embedded in TBS tissue freezing medium. Sections of 10-μm thickness were made with a cryostat at -20°C, placed on uncoated slides, and stained with hematoxylin and eosin. The stained slides were examined with a microscope (DMI 4000B), and the images were captured with a Fluotar 20× 0.4 NA dry objective (Leica) using a camera (RETIGA Exi). The images were processed as 24-bit colored images using Photoshop.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
skeletal muscle cell
Cellular Component
nucleus
Biological Context
Biological Process
myoblast fusion involved in skeletal muscle regeneration
Attribution
Names
Yuting Sun
Yejing Ge
Jenny Dmevich
Yong Zhao
Mark Band
Jie Chen
Published
JCB 189: 1157-1169, 2010
Pubmed
20566686
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13595
Archival Resource Key (ARK)
ark:/b7295/w9cil13595
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
bright-field microscopy
Parameters Imaged
absorption of illumination
Source of Contrast
differences in adsorption or binding of stain
compartmentalization of stain or label
Visualization Methods
hematoxylin
eosin
Processing History
Brightness and Contrast Adjusted
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
cryostat-sectioned tissue
Fix in liquid nitrogen–cooled 2-methylbutane
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1392px 0.32µm
Y 1040px 0.32µm


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