Alternate header for print version

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13599*  Cite 

Mouse hind limb tibialis anterior (TA) muscles were injected with BaCl2 followed by daily systemic administration of rapamycin (Rap) starting 7 days after injury. The animals were sacrificed at different days, and the injured muscles were dissected and cryosectioned followed by hematoxylin (blue) and eosin (pink to red) staining. This image shows the regeneration of muscle tissue fourteen days after the injury with daily systemic administration of Rap from day 7 to day 13 and a single dose of adenovirus expressing follistatin was injected into the injured TA muscle on day 7. The growth inhibition of myofibers by Rap was rescued by recombinant follistatin expression. Myofibers were stained by eosin in red and nuclei were stained by hematoxylin in blue. This image corresponds to Fig 10B from JCB 189: 1157-1169, 2010. See also CIL: 13595, 13596, 13597, 13598, and 13600.

Technical Details

TA muscles were isolated by dissection, frozen in liquid nitrogen–cooled 2-methylbutane, and embedded in TBS tissue freezing medium. Sections of 10-μm thickness were made with a cryostat at -20°C, placed on uncoated slides, and stained with hematoxylin and eosin. The stained slides were examined with a microscope (DMI 4000B), and the images were captured with a Fluotar 20× 0.4 NA dry objective (Leica) using a camera (RETIGA Exi). The images were processed as 24-bit colored images using Photoshop.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
skeletal muscle cell
Cellular Component
Biological Context
Biological Process
myoblast fusion involved in skeletal muscle regeneration
Molecular Function
mTOR inhibition
Yuting Sun
Yejing Ge
Jenny Dmevich
Yong Zhao
Mark Band
Jie Chen
JCB 189: 1157-1169, 2010
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
bright-field microscopy
Parameters Imaged
absorption of illumination
Source of Contrast
differences in adsorption or binding of stain
compartmentalization of stain or label
Visualization Methods
Processing History
Brightness and Contrast Adjusted
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
cryostat-sectioned tissue
Fix in liquid nitrogen–cooled 2-methylbutane
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 1392px 0.32µm
Y 1040px 0.32µm