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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13608*  Cite 
Description

Behavior of a patient-derived human fibroblast line (PD-NC4) containing a mutated chromosome 4 in which the normal centromere is epigenetically silenced and a neo-centromere has formed 25 Mbp down the q arm at a site that lacks centromere repeats. Shown is a z-series of the PD-NC4 chromosome from a metaphase spread stained for CENP-B (red), centromere (ACA, green) and DNA (DAPI blue). See Fig 1D in Bassett et al. 2010. Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors. J Cell Biol 190:177-185.

Technical Details

Cells were metaphase-arrested with nocodazole, swollen in 75 mM KCl, centrifuged onto glass slides, and fixed with formaldehyde before processing for indirect immunofluorescence. Digital z-series images were captured with an ORCA AG CCD mounted on DMI6000B Leica microscope using a 100X 1.4 NA objective lens and subsequently processed by deconvolution.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast
Cell Line
PD-NC4
Cellular Component
nuclear chromosome
chromosome, centromeric region
Biological Context
Biological Process
mitosis
Attribution
Names
Emily Bassett
Stacey Wood
Kevan Salimian
Sandya Ajith
Daniel Foltz
Ben Black
Published
Bassett et al. 2010 J Cell Biol 190:177-185
Pubmed
20643881
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13608
Archival Resource Key (ARK)
ark:/b7295/w9cil13608
Grouping This image is part of a group.
Imaging
Image Type
charge coupled device (CCD)
Image Mode
fluorescence emission
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
differences in adsorption or binding of stain
Visualization Methods
4',6-diamidino-2-phenylindole (DAPI)
Fluorescein (FITC)
Cy3
Cy5
Processing History
3D-deconvolution
Data Qualifiers
processed data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 101px ——
Y 91px ——
Z 20px ——