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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13723*  Cite 
Description

Expression of R98F PINK1-YFP (green), consistent with localization within mitochondria, does not induce mitochondrial translocation of mCherry-Parkin (red). The R98F mutation in PINK1-YFP partially inhibits proteolytic cleavage. Transfected HeLa cells were treated with DMSO for 1 hr. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4e, top row, and is a control for Fig 4e, bottom row of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
endothelial cell
Cell Line
HeLa
cervical carcinoma
Cellular Component
mitochondrion
cytosol
nucleus
mitochondrial inner membrane
mitochondrial outer membrane
Attribution
Names
Seok Min Jin
Michael Lazarou
Chunxin Wang
Lesley A. Kane
Derek P. Narendra
Richard J. Youle
Published
J Cell Biol, 191: 933-942, 2010.
Pubmed
21115803
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13723
Archival Resource Key (ARK)
ark:/b7295/w9cil13723
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EYFP
mCherryFP
Processing History
brightness and contrast adjusted
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.1395µm
Y 512px 0.1395µm