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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13726*  Cite 
Description

Expression of WT PINK1-YFP (green) does not induce mitochondrial translocation of mCherry-Parkin (red) in PINK1 knock-out mouse embryonic fibroblasts (PINK1 KO MEFs). Transfected PINK1 KO MEFs were treated with DMSO for 3 hrs. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Supplemental Figure S1c, top left row of J Cell Biol, 191: 933-942, 2010. Images in Supplemental Figure S1c include CIL#s 13725, 13726, 13727, 13728.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cellular Component
mitochondrion
cytosol
nucleus
mitochondrial inner membrane
mitochondrial outer membrane
Attribution
Names
Seok Min Jin
Michael Lazarou
Chunxin Wang
Lesley A. Kane
Derek P. Narendra
Richard J. Youle
Published
J Cell Biol, 191: 933-942, 2010.
Pubmed
21115803
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13726
Archival Resource Key (ARK)
ark:/b7295/w9cil13726
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EYFP
mCherryFP
Processing History
brightness and contrast adjusted
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.1395µm
Y 512px 0.1395µm