Smoothened (Smo; green), a downstream effector of Hedgehog (Hh), is expressed in most cells of the wild-type Drosophila germarium including follicle stem cells, but is excluded from Boi- and Hh-expressing apical cells (Hh, red). Germ cells are identified with anti-Vasa (blue). Image is Fig 4E in J Cell Biol. 2010. 191: 943-952.
Fly ovaries were dissected and fixed as described previously (O’Reilly et al., 2008). Primary antibodies were 1:2,000 rabbit anti-Vasa; 1:100 goat anti-Hh (Santa Cruz Biotechnology, Inc.) and 1:100 mouse anti-Smo (DSHB). Secondary antibodies used were FITC, Cy3, and Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).
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