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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13900*  Cite 

Saccharomyces cerevisiae (WT BY4741-MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0), transformed with EGFP-Atg8p, undergoing chronological aging on small synthetic 2% glucose media (SMD) without supplementation of spermidine. Image represents yeast morphology, visualized by DIC. DIC image was captured using a widefield microscope (Axioskop; Carl Zeiss, Inc) fitted with a Plan Neofluar 63× 1.25 NA oil immersion objective. Image acquired with a camera (SPOT 9.0 Monochrome 6; Diagnostic Instruments, Inc) using Metamorph software (Universal Imaging Corp) and processed with IrfanView and Photoshop (CS2; Adobe) software. Specifically, picture processing involved coloring and cropping of representative areas and was performed with IrfanView. In addition, linear adjustments of contrast and brightness were applied with Photoshop (using equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 2A, WT/Co/DIC panel, in Morselli et al. J Cell Biol 192: 615-629

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae S288c
Cell Line
BY 4741
Cellular Component
autophagic vacuole
Biological Context
Biological Process
Eugenia Morselli
Guillermo Mariño
Martin v. Bennetzen
Tobias Eisenberg
Evgenia Megalou
Sabrina Schroeder
Sandra Cabrera
Paule Bénit
Pierre Rustin
Alfredo Criollo
Oliver Kepp
Lorenzo Galluzzi
Shensi Shen
Shoaib Ahmad Malik
Maria Chiara Maiuri
Yoshiyuki Horio
Carlos López-Otín
Jens S. Andersen
Nektarios Tavernarakis
Frank Madeo
Guido Kroemer
J Cell Biol 192: 615-629
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
charge coupled device (CCD)
Image Mode
differential interference contrast microscopy
Parameters Imaged
optical path length gradient
Source of Contrast
boundaries between regions with different refractive index
Processing History
brightness and contrast adjusted
Data Qualifiers
processed data
Sample Preparation
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 1360px ——
Y 1024px ——