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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:14564*  Cite 

Polymerized actin localization in wild type Arabidopsis thaliana leaf trichomes, stage 4, during the transition to branch elongation. Detailed methods: Intact Arabidopsis shoots were fixed in 100 mM PIPES/KOH pH 6.9, 5 mM EGTA, 4 mM MgCl2 (PEM) containing 2 % formaldehyde. Actin filaments were detected with overnight incubation with 0.2 µM Alexa Fluor 488® phalloidin. Confocal images were obtained using a Biorad Radiance 2100 confocal head mounted on a Nikon E800 stand, excitation wavelength was the 488 line of a mixed gas Argon ion laser. The objective used was a 60X 1.2 NA water immersion lens, with optical zoom. Acquisition Parameters: Z-stack of confocal images of the actin cytoskeleton, 120 planes with a z-step size of 0.4 µm. Trichomes are unicellular structures that arise from single protodermal cells and usually form three highly elongated branches.

Biological Sources
NCBI Organism Classification
Arabidopsis thaliana
Cell Type
Cellular Component
actin cytoskeleton
Dan Szymanski, Purdue University
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 512px 0.196µm
Y 512px 0.196µm
Z 120px 0.4µm