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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:14676*  Cite 

The thylakoids of the vegetative cells of the filamentous cyanobacteria, Oscillatoria tenuis, are capable of oxygen-evolving photosynthesis. Freeze-fracture replicas of the vegetative cells revealed the structure of the thylakoids. The process of freeze-fracture splits memebranes along their hydrophobic interior revealing two complementary fracture faces designated P-face and E-face. Each fracture faces has a population of particles which represent intramembrane proteins or protein complexes. Oscillatoria cultures were maintained on agar plates. Cells were collected and suspended in 10 mL dH2O, spun at 2,000 g for 10 min in a clinical centrifuge, resuspended in 5 mL dH2O. Added 5 mL 70% glycerol over 1 hr with continuous stirring, spun at 2,000 g for 10 min in a clinical centrifuge. Pellets were placed on copper support discs, frozen in liquid Freon 12 and stored under liquid nitrogen. Replicas were prepared on a Blazers BA 360 freeze-etch unit and examined in a JEOL TEM 100C at 50,000X magnification.

Biological Sources
NCBI Organism Classification
Oscillatoria tenuis
Cellular Component
outer membrane
Biological Context
Biological Process
Thomas Giddings
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
Image Mode
transmission electron microscopy (TEM)
Source of Contrast
electron density
Visualization Methods
shadowing and plating
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 3437px 0.4nm
Y 4584px 0.4nm