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CIL:14969*  Cite 

Distribution of interneurons expressing EGFP from the 5HT3 receptor promoter (Tg(Htr3a-EGFP)DH30Gsat, colabelled for the CB1 cannabinoid receptor. This image is from the pyramidal cell layer in hippocampal CA1. Multiple EGFP+ bipolar interneurons are present within the pyramidal cell layer and a mesh of EGFP+ fibers and CB1+ axons is present around the pyramidal cell bodies, consistent with basket cell synapses. EGFP+ cells are also present in the stratum oriens and stratum radiatum. CB1 was detected with the L15 antibody (K. Mackie). Section (40 um) was imaged with a 32X, 0.4 NA Achroplan objective on a Zeiss Axiovert microscope. AF568 was excited at 562/40 nm and emission collected at 624/40 nm with Semrock filters and a single band dichroic (530-585 nm reflected, 601-800 nm transmitted). AF488 was excited at 482/35 nm and emission collected at 536/40 nm with Semrock filters and a single band dichroic (446-499 nm reflected, 513-735 nm transmitted). DAPI was excited at 350/50 nm and emission collected at 460/50 nm with a CLP400 dichroic (Chroma). Single images were captured for each channel using a Zeiss Axiocam MR CCD and pseudo-colored using Axiovision. See Davis and Puhl 2011 (Pubmed ID 21305052) for a detailed staining protocol. This image is part of a series characterizing EGFP expression from the 5HT3 promoter throughout the brain.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
CNS interneuron
pyramidal cell
basket cell
Cellular Component
neuronal cell body
Margaret I. Davis
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
vibratome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 1300px 0.35181496µm
Y 1030px 0.35181496µm