Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:150^* Cite

Description

Differential interference microscopy (DIC) image of PtK1 cell (marsupial kidney epithelial cell expressing dominant-negative Rac1(T17N). Live cells were used to study microtubule dynamics using X-rhodamine tubulin (injected at 1 mg/mL)and EGFP-Rac1(T17N) (expression vector injected into the cell nucleus at 100 ug/mL). Image corresponds to Fig 3 and microtubule dynamics correspond to Video 3 in J Cell Biol, 161:845-851, 2003. Video 3 is contained within the same image group as this figure.

Biological Sources

NCBI Organism Classification: Potorous tridactylus
Cell Type: epithelial cell
Cell Line: Pt K1 (NBL-3)
Cellular Component: cell; nucleus

Biological Context

Molecular Function: dominant negative Rac

Attribution

Names: Torsten Wittmann; Gary M. Bokoch; Clare M. Waterman-Storer
Published: J Cell Biol, 161:845-851, 2003
Pubmed: 12796474

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL150
Archival Resource Key (ARK): ark:/b7295/w9cil150

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1191px	——
Y	830px	——