Single-plane sequences of anucleate, centrosome-containing cytoplasts (sand dollars) three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). This anucleate cytoplast has four centrosomes variously spaced. A deeply ingressing furrow bisects the well-spaced centrosomes; shallow furrows form over closely spaced centrosomes. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. Video corresponds to Fig 7c and the third segment of video 6 in J Cell Biol. 2009 Dec 14;187(6):831-45.
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