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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:15810*  Cite 
Description

Purple sea urchin embryos undergoing mitosis. Embryos are expressing GFP-rhotekin GTPase binding domain, which is a probe for active Rho, with 3xmCherry-Ensconsin (a microtubule asociated protein shown in blue). Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C

Biological Sources
NCBI Organism Classification
Strongylocentrotus purpuratus
Cell Line
Embryo
Cellular Component
astral microtubule
spindle
microtubule
ensconsin
Biological Context
Biological Process
mitotic metaphase
mitotic anaphase
mitotic telophase
Molecular Function
active Rho
Attribution
Names
George Von Dassow
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL15810
Archival Resource Key (ARK)
ark:/b7295/w9cil15810
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
mCherryFP
Processing History
unprocessed raw data
Sample Preparation
Methods
unfixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 480px 0.21µm
Y 480px 0.21µm
Time 12 seconds 92